Print-capture PCR: a simple and highly sensitive method for the detection of plum pox virus (PPV) in plant tissues
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منابع مشابه
Print-capture PCR: a simple and highly sensitive method for the detection of plum pox virus (PPV) in plant tissues.
Plum pox virus (PPV) is the causal agent of sharka disease of stone fruit trees which is considered to be one of the most serious plant diseases. Sensitive techniques are needed for its diagnosis. Immunosorbent assays based on the use of monoclonal antibodies (1) and different PCR techniques have been described with or without previous immunocapture (2,3). In all instances plant extracts are ne...
متن کاملA highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection.
A highly sensitive assay, based on polymerase chain reaction amplification of cDNA synthesized from the viral RNA of antibody-captured viral particles, has been developed for plum pox potyvirus (PPV) detection. The reaction, called immunocapture/PCR (IC/PCR), yields a specific 243-bp product. The immunocapture step, by allowing the use of large sample volumes and by the viral particle prepurifi...
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Polymerase chain reaction (PCR) is a rapid and simple technique with high sensitivity and specificity. Inthe recent years, PCR has been used for rapid detection of viral nucleic acids, such as Humancytomegalovirus (HCMV), whereas, PCR optimization is an important task to be done, especially beforeit’s diagnostic application. Annealing temperature, ion concentration (especially...
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plum pox virus (ppv), the causal agent of sharka disease, belongs to the genus potyvirus in the family potyviridae and has been reported from some regions of iran. surveys were conducted to determine the incidence of the virus in khorasan razavi province in 2010 and 2011 and 553 samples of stone fruit trees were collected and tested by das-elisa. in this method 12 samples were infected with ppv...
متن کاملCloning of the complete infectious cDNA of the plum pox virus strain PPV-Rec.
UNLABELLED Plum pox virus (PPV) is the causal agent of Sharka, considered to be the most detrimental viral disease of Prunus spp. worldwide. So far, several PPV strains have been recognized, three of them (PPV-D, PPV-M, and PPV-Rec) having shown serious economic impact in the European area. Infectious cDNA clones of plant RNA viruses are excellent tools for functional studies of viral genomes. ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.11.2192